Mouse Anti-ACTA2 Recombinant Antibody (clone HHF35)
CAT#: ZG-1036F
This product is a mouse antibody that recognizes ACTA2.
Tested Data
Gene Expression
IF
Figure 1 Mouse Anti-ACTA2 Antibody (ZG-1036F) in IF
Indirect immunofluorescence staining of frozen section of chicken gizzard with anti-ACTA2 antibody (HHF35) showing specific positive staining of smooth muscle cells.
IHC
Figure 2 Mouse Anti-ACTA2 Antibody (ZG-1036F) in IHC
Formalin fixed, paraffin embedded human heart tissue immunostained for actin with anti-ACTA2 antibody (clone HHF35) at a 1:250 dilution.
IHC
Figure 3 Mouse Anti-ACTA2 Antibody (ZG-1036F) in IHC
Formalin fixed, paraffin embedded human small intestine, immunostained for actin using anti-ACTA2 antibody (clone HHF35) at a 1:100 dilution. Note staining of smooth muscle cells and no reactivity on the epithelium and connective tissue.
IHC
Figure 4 Mouse Anti-ACTA2 Antibody (ZG-1036F) in IHC
Formalin fixed, paraffin embedded human tongue tissue, immunostained for actin using anti-ACTA2 antibody (clone HHF35) at a 1:250 dilution. Note staining of striated muscle cells and no reactivity on the epithelium and connective tissue.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3761/630_C6_2_selected.jpg
Normal Tissue
Figure 2 Smooth muscle
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2/ihc_selected.jpg
Normal Tissue
Figure 3 Breast
Myoepithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_B_1_4.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_B_9_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13531/31344_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166, HHSteC, HSkMC, hTERT-RPE1, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107796-ACTA2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- HHF35 reacts with α-muscle and γ-smooth muscle actin, and therefore with skeletal muscle, cardiac muscle, blood vessel and visceral smooth muscle cells, pericytes and myoepithelial cells.
- Species Reactivity
- Chicken, Human, Monkey, Rabbit, Rat, Pig, Zebrafish
- Clone
- HHF35
- Applications
- Electron microscopy, ELISA, Immunocytochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Product Property
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% Sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Target
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No Non-Specific Binding
One of the best features is the lack of non-specific binding. This has improved the quality of our data significantly.
Robust and Reliable
This antibody has proven to be robust and reliable across different experimental conditions. Highly recommended!
Easy Sample Preparation
Sample preparation using this antibody is straightforward, and it works well with tissue as well as cell samples.
Q&As
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How do I address variability in antibody performance?
A: Use consistent protocols, validate new batches, and incorporate controls to monitor performance. Variability can stem from differences in sample preparation, assay conditions, or reagent quality.
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What is the role of secondary antibodies in detection?
A: Secondary antibodies bind to the primary antibody and are typically conjugated to detection molecules like enzymes or fluorophores, amplifying the signal for more sensitive detection.
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How can I confirm antibody binding specificity in my sample?
A: Perform competition assays, use isotype controls, and run parallel tests with known positive and negative controls to confirm specificity.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-1036F, RRID: AB_3112068)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "ACTA2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0048 | Hi-Affi™ Rabbit Anti-ACTA2 Recombinant Antibody (clone DS48AB) | IHC, ICC, FC | Rabbit IgG |
| MOR-4737 | Hi-Affi™ Rabbit Anti-ACTA2 Recombinant Antibody (clone TH251DS) | WB, IF, ICC, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0182C | Mouse Anti-ACTA2 Recombinant Antibody (clone 1E12) | WB, IHC-P, IF | Mouse IgG |
| ZG-0183C | Mouse Anti-ACTA2 Recombinant Antibody (clone 6A12) | WB, IF, IHC-P | Mouse IgG |
| VS3-QX11 | Mouse Anti-ACTA2 Recombinant Antibody (clone 4F4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX12 | Mouse Anti-ACTA2 Recombinant Antibody (clone 3F11-C2-G6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX13 | Mouse Anti-ACTA2 Recombinant Antibody (clone 7F12) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY109 | Anti-ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY110 | Anti-Mouse ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY111 | Anti-Rabbit ACTA2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY112 | Anti-Rat ACTA2 Immunohistochemistry Kit | IHC |
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